C - D

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Start/Produkter/Kontrollstammar/KWIK-STIK™ LYFO DISK®/C - D

Ready-to-use reference stock cultures will alleviate labor-intensive processes of preparing, storing, and maintaining in-house cultures. Each lyophilized microorganism is no more than four passages from the reference culture and is authentic and traceable. Choose from more than 750 microorganism strains derived from well-known culture collections from around the world including ATCC®, NCIMB, NCTC and more. Individual lyophilized microorganisms are available in LYFO DISK® and KWIK-STIK™ configurations. A Certificate of Quality comes standard with each shipment. Also, a printable Certificate of Analysis is available online at www.microbiologics.com 24 hours a day and 7 days a week.

*Note: Add the packaging option to the Catalog Number when placing an order: Cat # + L (for LYFO DISK® 6 Pack); Cat # + K (for KWIK-STIK™ 6 Pack); or Cat # + P (for KWIK-STIK™ 2 Pack). Examples are 5041L, 5041K or 5041P. For more information click on the information button for each individual strain.

Campylobacter coli ATCC® 33559™

01023K

Biosafety Level: 2

6 self-contained units of a single organism

Biosafety Level	2
Product Format	KWIK-STIK™ 6 Pack
Test Method	Food and Food Ingredients: Pathogen Testing, Microbial Identification
WDCM Number	00072
Catalog number	01023
Standards and Guidelines	FDA Bacteriological Analytical Manual (BAM)
Taxonomy	Bacteria
Industry Type	Food Safety
Media	Chocolate Agar
Temperature	35°C
Atmosphere	Microaerophilic
Growth Time	48 to 72 hours
Additional Tips	Note: Do not open the inoculated agar medium petri plate for the first 48 hours.

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Campylobacter coli ATCC® 33559™

01023L

Biosafety Level: 2

6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level	2
Product Format	LYFO DISK™
Test Method	Food and Food Ingredients: Pathogen Testing, Microbial Identification
WDCM Number	00072
Catalog number	01023
Standards and Guidelines	FDA Bacteriological Analytical Manual (BAM)
Taxonomy	Bacteria
Industry Type	Food Safety
Media	Chocolate Agar
Temperature	35°C
Atmosphere	Microaerophilic
Growth Time	48 to 72 hours
Additional Tips	Note: Do not open the inoculated agar medium petri plate for the first 48 hours.

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Campylobacter coli ATCC® 33559™

01023P

Biosafety Level: 2

2 self-contained units of a single organism

Biosafety Level	2
Product Format	KWIK-STIK™ 2 Pack
Test Method	Food and Food Ingredients: Pathogen Testing, Microbial Identification
WDCM Number	00072
Catalog number	01023
Standards and Guidelines	FDA Bacteriological Analytical Manual (BAM)
Taxonomy	Bacteria
Industry Type	Food Safety
Media	Chocolate Agar
Temperature	35°C
Atmosphere	Microaerophilic
Growth Time	48 to 72 hours
Additional Tips	Note: Do not open the inoculated agar medium petri plate for the first 48 hours.

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Desulfotomaculum nigrificans ATCC® 7946™*

01026K

Biosafety Level: 1

6 self-contained units of a single organism

Biosafety Level	1
Product Format	KWIK-STIK™ 6 Pack
Test Method	Microbial Identification
Catalog number	01026
Taxonomy	Bacteria
Media	Phase 1: ISF (modified Infant Soy Formula) Broth, Phase 2: Tranfer to Sulfite Agar
Temperature	Phase 1: 55°C, Phase 2: 55°C
Atmosphere	Phase 1: Anaerobic, Phase 2: Anaerobic
Growth Time	Phase 1: 48 hours, Phase 2: 48 hours to 7 days
Additional Tips	Prepare and Use ISF (modified Infant Soy Formula) Broth using the following steps: 1. Fill tubes with 10 ml Infant Soy Formula. Infant Soy Formula may be purchased at a grocery store. 2. Place a four-penny nail in each tube. A four-penny nail is approximately 1.5 inches, or 38 mm, in length. It should contain steel or iron. 3. Sterilize the broth. 4. Inoculate ISF Broth with one LYFO DISK® or KWIK-STIK™. 5. Grow at 55°C in anaerobic conditions for 48 hours. The broth will turn grey, indicating growth. 6. Make two dilutions, 1:10 and 1:100. 7. Sub with a swab to Sulfite Agar. Plate the undiluted sample and the 1:10 and 1:100 dilutions. It is necessary to plate the diluted samples because at higher concentrations the colonies are pin-point which makes colony characteristics difficult to see. Sulfite Agar is used for detecting thermophilic anaerobes which produce sulfite. 8. Incubate the agar in anaerobic environment at 55°C for 48 hours to 7 days.

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Desulfotomaculum nigrificans ATCC® 7946™*

01026L

Biosafety Level: 1

6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level	1
Product Format	LYFO DISK™
Test Method	Microbial Identification
Catalog number	01026
Taxonomy	Bacteria
Media	Phase 1: ISF (modified Infant Soy Formula) Broth, Phase 2: Tranfer to Sulfite Agar
Temperature	Phase 1: 55°C, Phase 2: 55°C
Atmosphere	Phase 1: Anaerobic, Phase 2: Anaerobic
Growth Time	Phase 1: 48 hours, Phase 2: 48 hours to 7 days
Additional Tips	Prepare and Use ISF (modified Infant Soy Formula) Broth using the following steps: 1. Fill tubes with 10 ml Infant Soy Formula. Infant Soy Formula may be purchased at a grocery store. 2. Place a four-penny nail in each tube. A four-penny nail is approximately 1.5 inches, or 38 mm, in length. It should contain steel or iron. 3. Sterilize the broth. 4. Inoculate ISF Broth with one LYFO DISK® or KWIK-STIK™. 5. Grow at 55°C in anaerobic conditions for 48 hours. The broth will turn grey, indicating growth. 6. Make two dilutions, 1:10 and 1:100. 7. Sub with a swab to Sulfite Agar. Plate the undiluted sample and the 1:10 and 1:100 dilutions. It is necessary to plate the diluted samples because at higher concentrations the colonies are pin-point which makes colony characteristics difficult to see. Sulfite Agar is used for detecting thermophilic anaerobes which produce sulfite. 8. Incubate the agar in anaerobic environment at 55°C for 48 hours to 7 days.

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Desulfotomaculum nigrificans ATCC® 7946™*

01026P

Biosafety Level: 1

2 self-contained units of a single organism

Biosafety Level	1
Product Format	KWIK-STIK™ 2 Pack
Test Method	Microbial Identification
Catalog number	01026
Taxonomy	Bacteria
Media	Phase 1: ISF (modified Infant Soy Formula) Broth, Phase 2: Tranfer to Sulfite Agar
Temperature	Phase 1: 55°C, Phase 2: 55°C
Atmosphere	Phase 1: Anaerobic, Phase 2: Anaerobic
Growth Time	Phase 1: 48 hours, Phase 2: 48 hours to 7 days
Additional Tips	Prepare and Use ISF (modified Infant Soy Formula) Broth using the following steps: 1. Fill tubes with 10 ml Infant Soy Formula. Infant Soy Formula may be purchased at a grocery store. 2. Place a four-penny nail in each tube. A four-penny nail is approximately 1.5 inches, or 38 mm, in length. It should contain steel or iron. 3. Sterilize the broth. 4. Inoculate ISF Broth with one LYFO DISK® or KWIK-STIK™. 5. Grow at 55°C in anaerobic conditions for 48 hours. The broth will turn grey, indicating growth. 6. Make two dilutions, 1:10 and 1:100. 7. Sub with a swab to Sulfite Agar. Plate the undiluted sample and the 1:10 and 1:100 dilutions. It is necessary to plate the diluted samples because at higher concentrations the colonies are pin-point which makes colony characteristics difficult to see. Sulfite Agar is used for detecting thermophilic anaerobes which produce sulfite. 8. Incubate the agar in anaerobic environment at 55°C for 48 hours to 7 days.

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Candida tropicalis ATCC® 1369™

01036K

Biosafety Level: 1

6 self-contained units of a single organism

Biosafety Level	1
Product Format	KWIK-STIK™ 6 Pack
Strain Characteristics	yeast cells
Test Method	Microbial Identification
Catalog number	01036
Taxonomy	Fungi
Media	Sabouraud Dextrose Emmons Agar
Temperature	25°C
Atmosphere	Aerobic
Growth Time	2 to 7 days
Additional Tips	Nonselective Sheep Blood Agar is an appropriate alternative. Note: Nutrient Agar, Tryptic Soy Agar, Potato Dextrose Agar, and Standard Methods Agar (Plate Count Agar) are appropriate alternatives together with an additional period (24 hours) of incubation.

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Candida tropicalis ATCC® 1369™*

01036L

Biosafety Level: 1

6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level	1
Product Format	LYFO DISK™
Strain Characteristics	yeast cells
Test Method	Microbial Identification
Catalog number	01036
Taxonomy	Fungi
Media	Sabouraud Dextrose Emmons Agar
Temperature	25°C
Atmosphere	Aerobic
Growth Time	2 to 7 days
Additional Tips	Nonselective Sheep Blood Agar is an appropriate alternative. Note: Nutrient Agar, Tryptic Soy Agar, Potato Dextrose Agar, and Standard Methods Agar (Plate Count Agar) are appropriate alternatives together with an additional period (24 hours) of incubation.

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Candida tropicalis ATCC® 1369™

01036P

Biosafety Level: 1

2 self-contained units of a single organism

Biosafety Level	1
Product Format	KWIK-STIK™ 2 Pack
Strain Characteristics	yeast cells
Test Method	Microbial Identification
Catalog number	01036
Taxonomy	Fungi
Media	Sabouraud Dextrose Emmons Agar
Temperature	25°C
Atmosphere	Aerobic
Growth Time	2 to 7 days
Additional Tips	Nonselective Sheep Blood Agar is an appropriate alternative. Note: Nutrient Agar, Tryptic Soy Agar, Potato Dextrose Agar, and Standard Methods Agar (Plate Count Agar) are appropriate alternatives together with an additional period (24 hours) of incubation.

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Corynebacterium urealyticum ATCC® 43044™*

01039K

Biosafety Level: 2

6 self-contained units of a single organism

Biosafety Level	2
Product Format	KWIK-STIK™ 6 Pack
Test Method	Microbial Identification
Catalog number	01039
Taxonomy	Bacteria
Media	Nonselective Sheep Blood Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 72 hours

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Corynebacterium urealyticum ATCC® 43044™*

01039L

Biosafety Level: 2

6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level	2
Product Format	LYFO DISK™
Test Method	Microbial Identification
Catalog number	01039
Taxonomy	Bacteria
Media	Nonselective Sheep Blood Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 72 hours

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Corynebacterium urealyticum ATCC® 43044™*

01039P

Biosafety Level: 2

2 self-contained units of a single organism

Biosafety Level	2
Product Format	KWIK-STIK™ 2 Pack
Test Method	Microbial Identification
Catalog number	01039
Taxonomy	Bacteria
Media	Nonselective Sheep Blood Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 72 hours

Välj

Corynebacterium renale ATCC® BAA-1785™*

01040K

Biosafety Level: 2

6 self-contained units of a single organism

Biosafety Level	2
Product Format	KWIK-STIK™ 6 Pack
Test Method	Microbial Identification
Catalog number	01040
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

Välj

Corynebacterium renale ATCC® BAA-1785™*

01040L

Biosafety Level: 2

6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level	2
Product Format	LYFO DISK™
Test Method	Microbial Identification
Catalog number	01040
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

Välj

Corynebacterium renale ATCC® BAA-1785™*

01040P

Biosafety Level: 2

2 self-contained units of a single organism

Biosafety Level	2
Product Format	KWIK-STIK™ 2 Pack
Test Method	Microbial Identification
Catalog number	01040
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Curtobacterium pusillum ATCC® 19096™*

01044K

Biosafety Level: 1

6 self-contained units of a single organism

Biosafety Level	1
Product Format	KWIK-STIK™ 6 Pack
Test Method	Microbial Identification
Catalog number	01044
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

Välj

Curtobacterium pusillum ATCC® 19096™*

01044L

Biosafety Level: 1

6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level	1
Product Format	LYFO DISK™
Test Method	Microbial Identification
Catalog number	01044
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

Välj

Curtobacterium pusillum ATCC® 19096™*

01044P

Biosafety Level: 1

2 self-contained units of a single organism

Biosafety Level	1
Product Format	KWIK-STIK™ 2 Pack
Test Method	Microbial Identification
Catalog number	01044
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Cellulosimicrobium cellulans ATCC® BAA-1816™*

01046K

Biosafety Level: 1

6 self-contained units of a single organism

Biosafety Level	1
Product Format	KWIK-STIK™ 6 Pack
Test Method	Microbial Identification
Catalog number	01046
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

Välj

Cellulosimicrobium cellulans ATCC® BAA-1816™*

01046L

Biosafety Level: 1

6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level	1
Product Format	LYFO DISK™
Test Method	Microbial Identification
Catalog number	01046
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

Välj

Cellulosimicrobium cellulans ATCC® BAA-1816™*

01046P

Biosafety Level: 1

2 self-contained units of a single organism

Biosafety Level	1
Product Format	KWIK-STIK™ 2 Pack
Test Method	Microbial Identification
Catalog number	01046
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

Välj

Cellulosimicrobium cellulans ATCC® BAA-1817™*

01047K

Biosafety Level: 1

6 self-contained units of a single organism

Biosafety Level	1
Product Format	KWIK-STIK™ 6 Pack
Test Method	Microbial Identification
Catalog number	01047
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

Välj

Cellulosimicrobium cellulans ATCC® BAA-1817™*

01047L

Biosafety Level: 1

6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level	1
Product Format	LYFO DISK™
Test Method	Microbial Identification
Catalog number	01047
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

Välj

Cellulosimicrobium cellulans ATCC® BAA-1817™*

01047P

Biosafety Level: 1

2 self-contained units of a single organism

Biosafety Level	1
Product Format	KWIK-STIK™ 2 Pack
Test Method	Microbial Identification
Catalog number	01047
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Clostridioides difficile derived from ATCC® BAA-1870™

01048K

6 self-contained units of a single organism

Biosafety Level	2
Product Format	KWIK-STIK™ 6 Pack
Strain Characteristics	Genotype: • tcdA positive, tcdB positive • cdtB positive Binary Toxin positive Toxinotype IIIb Ribotype 027 PFGE Type NAP1 REA type BI 8 Formerly Clostridium difficile
Test Method	Microbial Identification
Catalog number	01048
Taxonomy	Bacteria
Industry Type	Clinical
Media	Anaerobic Blood Agar
Temperature	35°C
Atmosphere	Anaerobic
Growth Time	48 to 72 hours
Additional Tips	Some obligate anaerobes may require 5 to 7 days to demonstrate sufficient growth.

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