50 ml in Infusion bottle 100 ml, blue felscap.
Also known as: Tryptone soy broth, Tryptic soy broth, Casein soya bean digest broth, Soybean-casein digest medium
Intended use: A general purpose nutritious broth capable of growing a wide range of bacteria and fungi. This medium is recommended by the USP and EP for the sterility testing of pharmaceutical products.
When the amount of air in the bottle is smaller than the amount of medium and the bottles are used for direct inoculation through the septum, it might be necessary to add oxygen. Due to the high carbohydrate level of TSB, the oxygen level will decrease in time.
Typical formula (g/l)*: Pancreatic digest of casein 17.0; Papaic digest of soya-bean (meal) 3.0; Sodium chloride 5.0; Dipotassium hydrogen phosphate/Dibasic potassium phosphate 2.5; Glucose monohydrate 2.5
Formula conform: latest version EP/USP
Interpretation: Growth in the medium is indicated by turbidity compared to an uninoculated control. Subculture to appropriate media for identification.
* Adjusted and/or supplemented as required to meet performance criteria