FTM + 3% Tween 80 + 0.3% Lecithin, 600 ml in Alpha bottle 1000 ml, red septum/silver screw cap.
Also known as: Fluid thioglycollate medium + 3% tween 80 + 0.3% lecithin
Intended use: A medium for sterility testing and the cultivation of anaerobic and aerobic organisms. Tween is an emulsifying agent.
Typical formula (g/l)*: L-Cystine 0.5; Agar 0.75; Sodium chloride 2.5; Glucose monohydrate 5.5; Yeast extract 5.0; Pancreatic digest of casein 15.0; Sodium thioglycollate 0.5; Resazurin sodium 0.001; Polysorbate 80 30.0; Lecithin 3.0
Interpretation: Growth in the medium is indicated by turbidity compared to an uninoculated control. Subculture to appropriate media for identification. Obligate anaerobes will grow only in that portion of the broth below the oxidized (pink-red) top layer.
* Adjusted and/or supplemented as required to meet performance criteria.