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Start/Products/Microorganisms/KWIK-STIK™ LYFO DISK®/T - Z

Ready-to-use reference stock cultures will alleviate labor-intensive processes of preparing, storing, and maintaining in-house cultures. Each lyophilized microorganism is no more than four passages from the reference culture and is authentic and traceable. Choose from more than 750 microorganism strains derived from well-known culture collections from around the world including ATCC®, NCIMB, NCTC and more. Individual lyophilized microorganisms are available in LYFO DISK® and KWIK-STIK™ configurations. A Certificate of Quality comes standard with each shipment. Also, a printable Certificate of Analysis is available online at www.microbiologics.com 24 hours a day and 7 days a week.

*Note: Add the packaging option to the Catalog Number when placing an order: Cat # + L (for LYFO DISK® 6 Pack); Cat # + K (for KWIK-STIK™ 6 Pack); or Cat # + P (for KWIK-STIK™ 2 Pack). Examples are 5041L, 5041K or 5041P. For more information click on the information button for each individual strain.

Zygosaccharomyces parabailii ATCC® MYA-4549™*

01011K

Biosafety Level: 1

6 self-contained units of a single organism

Biosafety Level	1
Product Format	KWIK-STIK™ 6 Pack
Strain Characteristics	yeast cells
Test Method	Microbial Identification
Catalog number	01011
Taxonomy	Fungi
Media	Sabouraud Dextrose Emmons Agar
Temperature	25°C
Atmosphere	Aerobic
Growth Time	2 to 7 days
Additional Tips	Note: Potato Dextrose Agar and Standard Methods Agar (Plate Count Agar) are appropriate alternatives together with an additional period (24 hours) of incubation.

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Zygosaccharomyces parabailii ATCC® MYA-4549™*

01011L

Biosafety Level: 1

6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level	1
Product Format	LYFO DISK™
Strain Characteristics	yeast cells
Test Method	Microbial Identification
Catalog number	01011
Taxonomy	Fungi
Media	Sabouraud Dextrose Emmons Agar
Temperature	25°C
Atmosphere	Aerobic
Growth Time	2 to 7 days
Additional Tips	Note: Potato Dextrose Agar and Standard Methods Agar (Plate Count Agar) are appropriate alternatives together with an additional period (24 hours) of incubation.

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Zygosaccharomyces parabailii ATCC® MYA-4549™*

01011P

Biosafety Level: 1

2 self-contained units of a single organism

Biosafety Level	1
Product Format	KWIK-STIK™ 2 Pack
Strain Characteristics	yeast cells
Test Method	Microbial Identification
Catalog number	01011
Taxonomy	Fungi
Media	Sabouraud Dextrose Emmons Agar
Temperature	25°C
Atmosphere	Aerobic
Growth Time	2 to 7 days
Additional Tips	Note: Potato Dextrose Agar and Standard Methods Agar (Plate Count Agar) are appropriate alternatives together with an additional period (24 hours) of incubation.

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Vibrio vulnificus ATCC® 27562™*

01076K

Biosafety Level: 2

6 self-contained units of a single organism

Biosafety Level	2
Product Format	KWIK-STIK™ 6 Pack
Test Method	Microbial Identification
WDCM Number	00139
Catalog number	01076
Taxonomy	Bacteria
Media	Tryptic Soy Agar: Consult Culture Additional Tips for hydration instructions.
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours
Additional Tips	Rehydrate in sterile Brain Heart Infusion Broth, Tryptic Soy Broth, or 0.85% Saline. Transfer a portion of hydrated material immediately to agar. Incubate aerobically at 35°C for 24 to 48 hours. Note: Rehydration with water may result in decreased or no recovery. Rehydration with fluid provided in the KWIK-STIK™ unit provides satisfactory recovery when grown on the recommended media.

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Vibrio vulnificus ATCC® 27562™*

01076L

Biosafety Level: 2

6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level	2
Product Format	LYFO DISK™
Test Method	Microbial Identification
WDCM Number	00139
Catalog number	01076
Taxonomy	Bacteria
Media	Tryptic Soy Agar: Consult Culture Additional Tips for hydration instructions.
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours
Additional Tips	Rehydrate in sterile Brain Heart Infusion Broth, Tryptic Soy Broth, or 0.85% Saline. Transfer a portion of hydrated material immediately to agar. Incubate aerobically at 35°C for 24 to 48 hours. Note: Rehydration with water may result in decreased or no recovery. Rehydration with fluid provided in the KWIK-STIK™ unit provides satisfactory recovery when grown on the recommended media.

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Vibrio vulnificus ATCC® 27562™*

01076P

Biosafety Level: 2

2 self-contained units of a single organism

Biosafety Level	2
Product Format	KWIK-STIK™ 2 Pack
Test Method	Microbial Identification
WDCM Number	00139
Catalog number	01076
Taxonomy	Bacteria
Media	Tryptic Soy Agar: Consult Culture Additional Tips for hydration instructions.
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours
Additional Tips	Rehydrate in sterile Brain Heart Infusion Broth, Tryptic Soy Broth, or 0.85% Saline. Transfer a portion of hydrated material immediately to agar. Incubate aerobically at 35°C for 24 to 48 hours. Note: Rehydration with water may result in decreased or no recovery. Rehydration with fluid provided in the KWIK-STIK™ unit provides satisfactory recovery when grown on the recommended media.

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Wallemia sebi ATCC® 42694™*

01141K

Biosafety Level: 1

6 self-contained units of a single organism

Biosafety Level	1
Product Format	KWIK-STIK™ 6 Pack
Strain Characteristics	Mold; formerly Wallemia sebi
Test Method	Media Testing, Microbial Identification
WDCM Number	00182
Catalog number	01141
Standards and Guidelines	ISO 11133
Taxonomy	Fungi
Industry Type	Food Safety
Media	Sabouraud Dextrose Emmons Agar
Temperature	25°C
Atmosphere	Aerobic
Growth Time	2 to 7 days

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Wallemia sebi ATCC® 42694™*

01141L

Biosafety Level: 1

6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level	1
Product Format	LYFO DISK™
Strain Characteristics	Mold; formerly Wallemia sebi
Test Method	Media Testing, Microbial Identification
WDCM Number	00182
Catalog number	01141
Standards and Guidelines	ISO 11133
Taxonomy	Fungi
Industry Type	Food Safety
Media	Sabouraud Dextrose Emmons Agar
Temperature	25°C
Atmosphere	Aerobic
Growth Time	2 to 7 days

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Wallemia sebi ATCC® 42694™*

01141P

Biosafety Level: 2

2 self-contained units of a single organism

Biosafety Level	1
Product Format	KWIK-STIK™ 2 Pack
Strain Characteristics	Mold; formerly Wallemia sebi
Test Method	Media Testing, Microbial Identification
WDCM Number	00182
Catalog number	01141
Standards and Guidelines	ISO 11133
Taxonomy	Fungi
Industry Type	Food Safety
Media	Sabouraud Dextrose Emmons Agar
Temperature	25°C
Atmosphere	Aerobic
Growth Time	2 to 7 days

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Yersinia enterocolitica derived from NCTC 11174

01213P

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Vibrio vulnificus derived from ATCC® 29307™*

01214K

Biosafety Level: 2

6 self-contained units of a single organism

Halophilic; Recommended by ISO 11133 for performance testing of culture media

Biosafety Level	2
Product Format	KWIK-STIK™ 6 Pack
Strain Characteristics	Halophilic Recommended by ISO 11133 for performance testing of culture media
Test Method	Media Testing, Microbial Identification
WDCM Number	00187
Catalog number	01214
Standards and Guidelines	ISO 11133
Taxonomy	Bacteria
Industry Type	Food Safety
Media	Tryptic Soy Agar: Consult Culture Additional Tips for hydration instructions.
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Vibrio vulnificus derived from ATCC® 29307™*

01214L

Biosafety Level: 2

6 lyophilized pellets of a single microorganism strain for quality control

Halophilic; Recommended by ISO 11133 for performance testing of culture media

Biosafety Level	2
Product Format	LYFO DISK™
Strain Characteristics	Halophilic\|Recommended by ISO 11133 for performance testing of culture media
Test Method	Media Testing, Microbial Identification
WDCM Number	00187
Catalog number	01214
Standards and Guidelines	ISO 11133
Taxonomy	Bacteria
Industry Type	Food Safety
Media	Tryptic Soy Agar: Consult Culture Additional Tips for hydration instructions.
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Vibrio vulnificus derived from ATCC® 29307™*

01214P

Biosafety Level: 2

2 self-contained units of a single organism

Halophilic; Recommended by ISO 11133 for performance testing of culture media

Biosafety Level	2
Product Format	KWIK-STIK™ 2 Pack
Strain Characteristics	Halophilic Recommended by ISO 11133 for performance testing of culture media
Test Method	Media Testing, Microbial Identification
WDCM Number	00187
Catalog number	01214
Standards and Guidelines	ISO 11133
Taxonomy	Bacteria
Industry Type	Food Safety
Media	Tryptic Soy Agar: Consult Culture Additional Tips for hydration instructions.
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Vibrio parahaemolyticus (o2:k3) derived from NCTC 10885

01236K

Biosafety Level: 2

6 self-contained units of a single organism

Serovar o2:k3

Biosafety Level	2
Product Format	KWIK-STIK™ 6 Pack
Strain Characteristics	Serovar o2:k3
Catalog number	01236
Taxonomy	Bacteria
Media	Tryptic Soy Agar: Consult Culture Additional Tips for hydration instructions.
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours
Additional Tips	Rehydrate in sterile Brain Heart Infusion Broth, Tryptic Soy Broth, or 0.85% Saline. Transfer a portion of hydrated material immediately to agar. Incubate aerobically at 35°C for 24 to 48 hours. Note: Rehydration with water may result in decreased or no recovery. Rehydration with fluid provided in the KWIK-STIK™ unit provides satisfactory recovery when grown on the recommended media.

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Vibrio parahaemolyticus (o2:k3) derived from NCTC 10885

01236L

Biosafety Level: 2

6 lyophilized pellets of a single microorganism strain for quality control

Serovar o2:k3

Biosafety Level	2
Product Format	LYFO DISK™
Strain Characteristics	Serovar o2:k3
Catalog number	01236
Taxonomy	Bacteria
Media	Tryptic Soy Agar: Consult Culture Additional Tips for hydration instructions.
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours
Additional Tips	Rehydrate in sterile Brain Heart Infusion Broth, Tryptic Soy Broth, or 0.85% Saline. Transfer a portion of hydrated material immediately to agar. Incubate aerobically at 35°C for 24 to 48 hours. Note: Rehydration with water may result in decreased or no recovery. Rehydration with fluid provided in the KWIK-STIK™ unit provides satisfactory recovery when grown on the recommended media.

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Vibrio parahaemolyticus (o2:k3) derived from NCTC 10885

01236P

Biosafety Level: 2

2 self-contained units of a single organism

Serovar o2:k3

Biosafety Level	2
Product Format	KWIK-STIK™ 2 Pack
Strain Characteristics	Serovar o2:k3
Catalog number	01236
Taxonomy	Bacteria
Media	Tryptic Soy Agar: Consult Culture Additional Tips for hydration instructions.
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours
Additional Tips	Rehydrate in sterile Brain Heart Infusion Broth, Tryptic Soy Broth, or 0.85% Saline. Transfer a portion of hydrated material immediately to agar. Incubate aerobically at 35°C for 24 to 48 hours. Note: Rehydration with water may result in decreased or no recovery. Rehydration with fluid provided in the KWIK-STIK™ unit provides satisfactory recovery when grown on the recommended media.

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Talaromyces pinophilus derived from ATCC® 11797™

01238K

Biosafety Level: 1

6 self-contained units of a single organism

Biosafety Level	1
Product Format	KWIK-STIK™ 6 Pack
Strain Characteristics	Mold, Fungus resistance testing, formerly Penicillium funiculosum. The strain was re-identified as Talaromyces pinophilus (syn. Penicillium pinophilum) based on the multiloci DNA sequence analysis.
Test Method	Microbial Identification
Catalog number	01238
Taxonomy	Fungi
Media	Sabouraud Dextrose Emmons Agar
Temperature	25°C
Atmosphere	Aerobic
Growth Time	2 to 7 days
Additional Tips	Nonselective Sheep Blood Agar is an appropriate alternative. Note: Nutrient Agar, Tryptic Soy Agar, Potato Dextrose Agar, and Standard Methods Agar (Plate Count Agar) are appropriate alternatives together with an additional period (24 hours) of incubation.

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Talaromyces pinophilus derived from ATCC® 11797™

01238L

Biosafety Level: 1

6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level	1
Product Format	LYFO DISK™
Strain Characteristics	Mold, Fungus resistance testing, formerly Penicillium funiculosum. The strain was re-identified as Talaromyces pinophilus (syn. Penicillium pinophilum) based on the multiloci DNA sequence analysis.
Test Method	Microbial Identification
Catalog number	01238
Taxonomy	Fungi
Industry Type	Cannabis
Media	Sabouraud Dextrose Emmons Agar
Temperature	25°C
Atmosphere	Aerobic
Growth Time	2 to 7 days
Additional Tips	Nonselective Sheep Blood Agar is an appropriate alternative. Note: Nutrient Agar, Tryptic Soy Agar, Potato Dextrose Agar, and Standard Methods Agar (Plate Count Agar) are appropriate alternatives together with an additional period (24 hours) of incubation.

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Talaromyces pinophilus derived from ATCC® 11797™

01238P

Biosafety Level: 1

2 self-contained units of a single organism

Biosafety Level	1
Product Format	KWIK-STIK™ 2 Pack
Strain Characteristics	Mold, Fungus resistance testing, formerly Penicillium funiculosum. The strain was re-identified as Talaromyces pinophilus (syn. Penicillium pinophilum) based on the multiloci DNA sequence analysis.
Test Method	Microbial Identification
Catalog number	01238
Taxonomy	Fungi
Media	Sabouraud Dextrose Emmons Agar
Temperature	25°C
Atmosphere	Aerobic
Growth Time	2 to 7 days
Additional Tips	Nonselective Sheep Blood Agar is an appropriate alternative. Note: Nutrient Agar, Tryptic Soy Agar, Potato Dextrose Agar, and Standard Methods Agar (Plate Count Agar) are appropriate alternatives together with an additional period (24 hours) of incubation.

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Ureaplasma urealyticum ATCC® 27813™ *

0151L

Biosafety Level: 2

6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level	2
Product Format	LYFO DISK™
Test Method	Microbial Identification
Catalog number	0151
Taxonomy	Bacteria
Media	See note below for important direction. Phase 1: SP4 with Urea Broth, Phase 2: A8 Agar
Temperature	Phase 1: 35°C, Phase 2: 35°C
Atmosphere	Phase 1: Aerobic, Phase 2: Anaerobic
Growth Time	Phase 1: 24 to 96 hours, Phase 2: 4 to 6 days
Additional Tips	Note: Inoculate Broth with LYFO DISK™. For Phase 1, prepare a 1:10 serial dilution using SP4 Urea Broth. Incubate the broth aerobically at 35°C for 24 to 96 hours. As soon as the SP4 Urea Broth turns red, begin Phase 2 by plating 0.1 ml of the broth to A8 Agar and streak for isolation. Incubate A8 agar anaerobically at 35°C for 4 to 6 days. Do not use cotton swabs or wooden sticks. In order to see colonies, examine plates microscopically.

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Zygosaccharomyces rouxii NCYC 381

0255K

Biosafety Level: 1

6 self-contained units of a single organism

Biosafety Level	1
Product Format	KWIK-STIK™ 6 Pack
Test Method	Microbial Identification
Catalog number	0255
Taxonomy	Bacteria
Media	Sabouraud Dextrose Emmons Agar
Temperature	25°C
Atmosphere	Aerobic
Growth Time	2 to 7 days
Additional Tips	Nonselective Sheep Blood Agar is an appropriate alternative. Note: Nutrient Agar, Tryptic Soy Agar, Potato Dextrose Agar, and Standard Methods Agar (Plate Count Agar) are appropriate alternatives together with an additional period (24 hours) of incubation.

Choose

Zygosaccharomyces rouxii NCYC 381

0255L

Biosafety Level: 1

6 lyophilized pellets of a single microorganism strain for quality control

Biosafety Level	1
Product Format	LYFO DISK™
Test Method	Microbial Identification
Catalog number	0255
Taxonomy	Bacteria
Media	Sabouraud Dextrose Emmons Agar
Temperature	25°C
Atmosphere	Aerobic
Growth Time	2 to 7 days
Additional Tips	Nonselective Sheep Blood Agar is an appropriate alternative. Note: Nutrient Agar, Tryptic Soy Agar, Potato Dextrose Agar, and Standard Methods Agar (Plate Count Agar) are appropriate alternatives together with an additional period (24 hours) of incubation.

Choose

Zygosaccharomyces rouxii NCYC 381

0255P

Biosafety Level: 1

2 self-contained units of a single organism

Biosafety Level	1
Product Format	KWIK-STIK™ 2 Pack
Test Method	Microbial Identification
Catalog number	0255
Taxonomy	Bacteria
Media	Sabouraud Dextrose Emmons Agar
Temperature	25°C
Atmosphere	Aerobic
Growth Time	2 to 7 days
Additional Tips	Nonselective Sheep Blood Agar is an appropriate alternative. Note: Nutrient Agar, Tryptic Soy Agar, Potato Dextrose Agar, and Standard Methods Agar (Plate Count Agar) are appropriate alternatives together with an additional period (24 hours) of incubation.

Choose

Yersinia enterocolitica subsp. enterocolitica ATCC® 23715™ *

0316K

Biosafety Level: 2

6 self-contained units of a single organism

biotype 1; serotype 8

Biosafety Level	2
Product Format	KWIK-STIK™ 6 Pack
Strain Characteristics	biotype 1 serotype 8
Test Method	Media Testing, Microbial Identification
WDCM Number	00160
Catalog number	0316
Standards and Guidelines	ISO 11133
Taxonomy	Bacteria
Industry Type	Food Safety
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Yersinia enterocolitica subsp. enterocolitica ATCC® 23715™*

0316L

Biosafety Level: 2

6 lyophilized pellets of a single microorganism strain for quality control

biotype 1; serotype 8

Biosafety Level	2
Product Format	LYFO DISK™
Strain Characteristics	biotype 1 serotype 8
Test Method	Media Testing, Microbial Identification
WDCM Number	00160
Catalog number	0316
Standards and Guidelines	ISO 11133
Taxonomy	Bacteria
Industry Type	Food Safety
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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