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Start/Products/Microorganisms/KWIK-STIK™ PLUS/KWIK-STICK™ PLUS A-L

KWIK-STIK™ PLUS A-L

MicroBioLogics® offers products designed to enhance your laboratory's quality control program by adding efficiency and reliability. KWIK-STIK™ Plus microorganisms are designed to meet the needs of laboratories requiring QC microorganisms that are a limited number of passages from the reference culture. KWIK-STIK™ Plus microorganisms are two passages from the reference culture and come in canisters containing five units of a single strain. KWIK-STIK™ Plus microorganisms have the same characteristics as the KWIK-STIK™ with two additional features:
- KWIK-STIK™ Plus microorganisms are lyophilized microorganism preparations that are two passages from the reference culture.
- A peel-off label contains a Certificate of Assay that specifies the identity and traceability of the microorganism to a reference culture, and the number of passages the microorganism preparation has been removed from the reference culture.

Brevibacillus agri ATCC® 51663™*

0139X

Biosafety Level: 1

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Biosafety Level	1
Product Format	KWIK-STIK™ Plus
Test Method	Microbial Identification
Catalog number	0139
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Lactobacillus leichmannii ATCC® 7830™*

0235X

Biosafety Level: 1

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Formerly Lactobacillus delbrueckii subsp. lactis

Biosafety Level	1
Product Format	KWIK-STIK™ Plus
Strain Characteristics	formerly Lactobacillus delbrueckii subsp. lactis
Test Method	Microbial Identification
Catalog number	0235
Taxonomy	Bacteria
Media	Phase 1: MRS (Man, Rogosa, Sharpe) Broth. Phase 2: Transfer to Columbia CNA with Sheep Blood or Tryptic Soy Agar with Sheep Blood
Temperature	Phase 1: 35°C Phase 2: 35°C
Atmosphere	Phase 1: Aerobic. Phase 2: 5 to 7% Carbon Dioxide
Growth Time	Phase 1: 48 hours. Phase 2: 48 hours
Additional Tips	Note: For Phase 1, the primary growth medium is MRS (Man, Rogosa, Sharpe) Broth. Incubate at 35°C in aerobic atmosphere for 48 hours. For Phase 2, transfer to either Columbia CNA with Sheep Blood or Tryptic Soy Agar with Sheep Blood. Incubate at 35°C in 5 to 7% carbon dioxide for 48 hours.

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Bacillus cereus ATCC® 11778™*

0256X

Biosafety Level: 1

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Biosafety Level	1
Product Format	KWIK-STIK™ Plus
Test Method	Microbial Identification
WDCM Number	00001
Catalog number	0256
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours
Additional Tips	Note: Some Bacillus spp. demonstrate better recovery on subculture when the stock microorganism growth is maintained at room temperature rather than 2°C to 8°C.

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Bacillus pumilus ATCC® 14884 ™ *

0258X

Biosafety Level: 1

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Biosafety Level	1
Product Format	KWIK-STIK™ Plus
Test Method	Microbial Identification
Catalog number	0258
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours
Additional Tips	Note: Some Bacillus spp. demonstrate better recovery on subculture when the stock microorganism growth is maintained at room temperature rather than 2°C to 8°C.

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Klebsiella aerogenes derived from ATCC® 13048™

0306X

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Biosafety Level	1
Product Format	KWIK-STIK™ Plus
Strain Characteristics	Formerly Enterobacter aerogenes
Test Method	Microbial Identification
WDCM Number	00175
Catalog number	0306
Taxonomy	Bacteria
Industry Type	Pharmaceutical
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Clostridium sporogenes ATCC® 19404 ™ *

0317X

Biosafety Level: 1

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Biosafety Level	1
Product Format	KWIK-STIK™ Plus
Test Method	Microbial Identification
WDCM Number	00008
Catalog number	0317
Standards and Guidelines	European Pharmacopoeia (Ph. Eur.), Japanese Pharmacopoeia (JP), U.S. Pharmacopeia (USP)
Taxonomy	Bacteria
Industry Type	Pharmaceutical
Media	Anaerobic Blood Agar
Temperature	35°C
Atmosphere	Anaerobic
Growth Time	48 to 72 hours
Additional Tips	Note: Nutrient Agar, Tryptic Soy Agar (Soybean Casein Digest Agar), and Standard Methods Agar (Plate Count Agar) are appropriate alternatives of some Clostridium species together with an additional period (24 hours) of incubation. Clostridium species may have reduced recovery when using the alternative agars.

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Candida albicans derived from ATCC® 14053™*

0332X

Biosafety Level: 1

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Biosafety Level	1
Product Format	KWIK-STIK™ Plus
Strain Characteristics	yeast cells
Test Method	Microbial Identification
Catalog number	0332
Taxonomy	Fungi
Media	Sabouraud Dextrose Emmons Agar
Temperature	25°C
Atmosphere	Aerobic
Growth Time	2 to 7 days
Additional Tips	Nonselective Sheep Blood Agar is an appropriate alternative. Note: Nutrient Agar, Tryptic Soy Agar, Potato Dextrose Agar, and Standard Methods Agar (Plate Count Agar) are appropriate alternatives together with an additional period (24 hours) of incubation.

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Escherichia coli ATCC® 25922 ™ *

0335X

Biosafety Level: 1

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

CLSI and EUCAST control for antimicrobial susceptibility testing

Biosafety Level	1
Product Format	KWIK-STIK™ Plus
Test Method	Microbial Identification
WDCM Number	00013
Catalog number	0335
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Klebsiella pneumoniae subsp. pneumoniae ATCC® 13883 ™ *

0351X

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Biosafety Level	2
Product Format	KWIK-STIK™ Plus
Strain Characteristics	Methyl Red positive Voges-Proskauer negative Thermotolerant
Test Method	Microbial Identification
Catalog number	0351
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Enterococcus faecalis ATCC® 29212 ™ *

0366X

Biosafety Level: 2

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

CLSI and EUCAST control control for antimicrobial susceptibility testing

Biosafety Level	2
Product Format	KWIK-STIK™ Plus
Test Method	Microbial Identification
WDCM Number	00087
Catalog number	0366
Taxonomy	Bacteria
Industry Type	Pharmaceutical

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Aspergillus brasiliensis ATCC® 16404 ™ *

0392X

Biosafety Level: 1

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

fungus

Biosafety Level	1
Product Format	KWIK-STIK™ Plus
Test Method	Microbial Identification
WDCM Number	00053
Catalog number	0392
Standards and Guidelines	European Pharmacopoeia (Ph. Eur.), ISO 11930, Japanese Pharmacopoeia (JP), Personal Care Product Council (PCPC) Microbiology Guidelines, U.S. Pharmacopeia (USP), U.S. Pharmacopeia (USP) - Dietary Supplements
Taxonomy	Bacteria
Industry Type	Personal Care Products/Cosmetics, Pharmaceutical
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Candida albicans ATCC® 10231 ™ *

0443X

Biosafety Level: 1

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Biosafety Level	1
Product Format	KWIK-STIK™ Plus
Strain Characteristics	Yeast cells
Test Method	Microbial Identification
WDCM Number	00054
Catalog number	0443
Standards and Guidelines	European Pharmacopoeia (Ph. Eur.), ISO 11930, Japanese Pharmacopoeia (JP), Personal Care Product Council (PCPC) Microbiology Guidelines, U.S. Pharmacopeia (USP), U.S. Pharmacopeia (USP) - Dietary Supplements
Taxonomy	Fungi
Industry Type	Nutraceutical/Dietary Supplements, Personal Care Products/Cosmetics, Pharmaceutical

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Escherichia coli ATCC® 8739 ™ *

0483X

Biosafety Level: 1

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Biosafety Level	1
Product Format	KWIK-STIK™ Plus
Test Method	Microbial Identification
WDCM Number	00012
Catalog number	0483
Standards and Guidelines	Environmental Protection Agency (EPA), European Pharmacopoeia (Ph. Eur.), ISO 11133, ISO 11930, ISO 21149, Japanese Pharmacopoeia (JP), Personal Care Product Council (PCPC) Microbiology Guidelines, U.S. Pharmacopeia (USP), U.S. Pharmacopeia (USP) - Dietary Supplements
Taxonomy	Bacteria
Industry Type	Food Safety, Nutraceutical/Dietary Supplements, Personal Care Products/Cosmetics, Water and Environmental
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Bacillus spizizenii derived from ATCC® 6633™

0486X

Biosafety Level: 1

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Biosafety Level	1
Product Format	KWIK-STIK™ Plus
Test Method	Antibiotic-Microbial Assays, Microbial Identification
WDCM Number	00003
Catalog number	0486
Standards and Guidelines	European Pharmacopoeia (Ph. Eur.), Japanese Pharmacopoeia (JP), U.S. Pharmacopeia (USP)
Taxonomy	Bacteria
Industry Type	Pharmaceutical
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours
Additional Tips	Note: Some Bacillus spp. demonstrate better recovery on subculture when the stock microorganism growth is maintained at room temperature rather than 2°C to 8°C.

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Clostridium sporogenes ATCC® 11437 ™ *

0487X

Biosafety Level: 1

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Biosafety Level	1
Product Format	KWIK-STIK™ Plus
Test Method	Microbial Identification
Catalog number	0487
Standards and Guidelines	European Pharmacopoeia (Ph. Eur.), Japanese Pharmacopoeia (JP), U.S. Pharmacopeia (USP)
Taxonomy	Bacteria
Industry Type	Pharmaceutical
Media	Anaerobic Blood Agar
Temperature	35°C
Atmosphere	Anaerobic
Growth Time	48 to 72 hours
Additional Tips	Note: Nutrient Agar, Tryptic Soy Agar (Soybean Casein Digest Agar), and Standard Methods Agar (Plate Count Agar) are appropriate alternatives of some Clostridium species together with an additional period (24 hours) of incubation. Clostridium species may have reduced recovery when using the alternative agars.

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Burkholderia cepacia ATCC® 25416 ™ *

0488X

Biosafety Level: 2

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Biosafety Level	2
Product Format	KWIK-STIK™ Plus
Test Method	Antimicrobial Effectiveness/Preservation Efficacy Testing, Microbial Identification
Catalog number	0488
Standards and Guidelines	Personal Care Product Council (PCPC) Microbiology Guidelines
Taxonomy	Bacteria
Industry Type	Personal Care Products/Cosmetics
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Aspergillus niger ATCC® 6275 ™ *

0500X

Biosafety Level: 1

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

fungus

Biosafety Level	1
Product Format	KWIK-STIK™ Plus
Strain Characteristics	Mold
Test Method	Microbial Identification
Catalog number	0500
Taxonomy	Fungi
Media	Sabouraud Dextrose Emmons Agar
Temperature	25°C
Atmosphere	Aerobic
Growth Time	2 to 7 days
Additional Tips	Nonselective Sheep Blood Agar is an appropriate alternative. Note: Nutrient Agar, Tryptic Soy Agar, Potato Dextrose Agar, and Standard Methods Agar (Plate Count Agar) are appropriate alternatives together with an additional period (24 hours) of incubation.

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Haemophilus influenzae ATCC® 49247™*

0647X

Biosafety Level: 2

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Reduced susceptibility to beta-lactam agents due to PBP mutations in Haemophilus influenzae;
Ampicillin resistant;
CLSI and EUCAST control for antimicrobial susceptibility testing

Biosafety Level	2
Product Format	KWIK-STIK™ Plus
Strain Characteristics	Reduced susceptivility to beta-lactam agents due to PBP mutations in Haemophilus influenzae Ampicillin resistant CLSI and EUCAST control for antimicrobial susceptibility testing
Catalog number	0647
Standards and Guidelines	Clinical Laboratory Standards Institute (CLSI), European Committee on Antimicrobial Susceptibility Testing (EUCAST)
Taxonomy	Bacteria
Industry Type	Clinical
Media	Chocolate Agar
Temperature	35°C
Atmosphere	5 to 7% Carbon Dioxide
Growth Time	24 to 48 hours

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Enterococcus hirae ATCC® 8043™*

0650X

Biosafety Level: 1

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Biosafety Level	1
Product Format	KWIK-STIK™ Plus
Test Method	Microbial Identification
Catalog number	0650
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Bordetella bronchiseptica ATCC® 4617 ™ *

0671X

Biosafety Level: 2

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Biosafety Level	2
Product Format	KWIK-STIK™ Plus
Test Method	Antibiotic-Microbial Assays, Microbial Identification
Catalog number	0671
Standards and Guidelines	U.S. Pharmacopeia (USP)
Taxonomy	Bacteria
Industry Type	Pharmaceutical
Media	Chocolate agar, Sheep Blood Agar, Tryptic Soy Agar, or Bordet Gengou Agar with 15% Defibrinated Sheep Blood
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours
Additional Tips	Note: Standard Methods Agar (Plate Count Agar) or Nutrient Agar are appropriate alternatives together with an additional period (24 hours) of incubation.

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Enterococcus hirae ATCC® 10541 ™ *

0678X

Biosafety Level: 1

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Biosafety Level	1
Product Format	KWIK-STIK™ Plus
Test Method	Microbial Identification
WDCM Number	00011
Catalog number	0678
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Klebsiella pneumoniae subsp. pneumoniae ATCC® 4352™*

0683X

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Biosafety Level	2
Product Format	KWIK-STIK™ Plus
Strain Characteristics	Thermotolerant
Test Method	Microbial Identification
Catalog number	0683
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Klebsiella pneumoniae subsp. pneumoniae ATCC® 10031 ™ *

0684X

Biosafety Level: 2

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Methyl Red positive, Voges-Proskauer negative

Biosafety Level	2
Product Format	KWIK-STIK™ Plus
Strain Characteristics	Methyl Red positive, Voges-Proskauer negative
Test Method	Antimicrobial Effectiveness/Preservation Efficacy Testing, Microbial Identification
Catalog number	0684
Standards and Guidelines	Personal Care Product Council (PCPC) Microbiology Guidelines
Taxonomy	Bacteria
Industry Type	Personal Care Products/Cosmetics
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Listeria monocytogenes ATCC® 19115 ™ *

0687X

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Biosafety Level	2
Product Format	KWIK-STIK™ Plus
Strain Characteristics	serotype 4b
Test Method	Microbial Identification
Catalog number	0687
Taxonomy	Bacteria
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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Kocuria rhizophila ATCC® 9341 ™ *

0688X

Biosafety Level: 1

5 KWIK-STIK™ units with a microorganism limited to 2 passages from the reference culture.

Biosafety Level	1
Product Format	KWIK-STIK™ Plus
Test Method	Antibiotic-Microbial Assays, Microbial Identification
Catalog number	0688
Standards and Guidelines	U.S. Pharmacopeia (USP)
Taxonomy	Bacteria
Industry Type	Pharmaceutical
Media	Tryptic Soy Agar (Soybean Casein Digest Agar), Non-selective Sheep Blood Agar, Standard Methods Agar (Plate Count Agar) or Nutrient Agar
Temperature	35°C
Atmosphere	Aerobic
Growth Time	24 to 48 hours

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KWIK-STIK™ PLUS A-L

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