Rapid Perfringens Medium, 10 ml in tube 14 ml, snap cap.
Also known as: RPM
Intended use: Selective enumeration of Clostridium perfringens.
Typical formula (g/l)*: L-Cystine 0.5; Agar 0.75; Sodium chloride 4.0; Glucose monohydrate 10.5; Yeast extract 8.0; Pancreatic digest of casein 15.0; Sodium thioglycollate 0.5; Resazurin sodium 0.001; Peptone 5.0; Gelatin 60.0; Dipotassium hydrogen phosphate 5.0; Iron(II)sulfate 0.5; Litmus milk 70.0; Neomycin 0.075; Polymyxine 0.0125
Interpretation: Clostridium perfringens causes a stormy fermentation characterized by coagulation of milk, gas formation and fracturing of the clot into a spongy mass. Presumptive positive tubes should be confirmed.
* Adjusted and/or supplemented as required to meet performance criteria.