Brain Heart Infusion (BHI) is a general-purpose liquid medium used in the cultivation of fastidious and K 100. nonfastidious microorganisms, including aerobic and anaerobic bacteria, from a variety of clinical andnonclinical materials. It serves as a base for supplemented media containing 0.1% agar, Fildes enrichment,or 6.5% sodium chloride. A supplemented pre-reduced formulation in tubes is especially recommended forthe cultivation of anaerobes.