Demi-Fraser Broth, 10 ml in plastic tube 20 ml, plastic swab in medium.
Intended use: Enrichment broth for the isolation of Listeria spp.
Typical formula (g/l)*: Meat peptone 5.0; Tryptone 5.0; Beef extract 5.0; Yeast extract 5.0; Sodium chloride 20.0; Disodium hydrogen phosphate dihydrate 12.0; Potassium dihydrogen phosphate 1.35; Aesculin 1.0; Lithium chloride 3.0; Nalidixic acid 10.0 mg; Acriflavine 12.5 mg; Ferric ammonium citrate 0.5
Formula conform: ISO 11290-1
Interpretation: Blackening of the broth indicates the presence of a potential Listeria, but regardless of the colour, it should be subcultured further.
*Adjusted and/or supplemented as required to meet performance criteria.